Beneficial Effects of Prebiotic Saccharomyces cerevisiae Mannan on Allergic Asthma Mouse Models

Figure 2

Effect of SC-MN on OVA-induced airway pathology in BALB/c mice. Upper panels: OVA-allergic BALB/c mouse. Lower panels: SC-MN-treated allergic BALB/c mouse (1 mg, i.n., 30 min prior to each OVA challenge). (a) H&E staining of the trachea shows epithelial denudation (arrow) compared to that of the intact epithelium in SC-MN-treated mouse (b). (c–f) FRI of mice 30 min (c, d) and 24 hrs (e, f) post i.n. administration of liposome. Notice the increase in central airway signals (arrow head) with less lung deposition (c) and faster clearance (arrow) (e) in untreated allergic mouse compared to the SC-MN-treated mouse (d, f). Images of the abdomen showed no signals in the stomach. Color intensity: red > yellow > green > blue. The FRI images are representative of 2 separate experiments with similar results. (g) Peribronchial cellular infiltration in H&E lung sections compared to the paucity of peribronchial cellular infiltration in SC-MN-treated mouse (h), representative of 3 separate experiments. Final magnification: 100x.