HLA Class Ia and Ib Polyreactive Anti-HLA-E IgG2a Monoclonal Antibodies (TFL-006 and TFL-007) Suppress Anti-HLA IgG Production by CD19+ B Cells and Proliferation of CD4+ T Cells While Upregulating Tregs

Figure 7

Dose-dependent inhibition of PHA-activated CD4+/CD8+ T cells in vitro with anti-HLA-E mAbs, TFL-007s (“s” for culture supernatants). The T cells were stained with PE-labeled anti-CD4 mAbs (x-axis) and PerCP-labeled anti-CD8 mAbs (y-axis). The profile is divided into three groups (only group 3 is represented in the figure; for details, see [47]) based on staining and size of cells to illustrate the differences in the CD4+ and CD8+ T cell populations and number of events. Group 1 comprises resting CD4+ and CD8+ lymphocytes, group 2 resting CD4+ and CD8+ lymphocytes, and group 3 CD4+ and CD8+ lymphoblasts. Flow cytometric profiles of PHA-treated CD4+ T cells (lower right of the boxes) and CD8+ T cells (upper left) from a normal non-alloimmunized donor (R) after treatment with mAb TFL-007s. The top row (treated only with PHA) shows the number of CD4+ T cells and the CD8+ T cells. The middle row (PHA and mAb TFL-007s at 1/10 dilution or 5 μg/ml) shows the number of both CD4+ () and CD8+ () T cells have decreased significantly. In comparison, the bottom row with the same treatment, but at 1/100 dilution (0.5 μg/ml), showed a dose-dependent decrease in the number of PHA-activated CD4+ () and CD8+ (not significant) T lymphocytes. Each block of figure represents one of the triplicate analyses (for further details, see [47]).