HLA Class Ia and Ib Polyreactive Anti-HLA-E IgG2a Monoclonal Antibodies (TFL-006 and TFL-007) Suppress Anti-HLA IgG Production by CD19+ B Cells and Proliferation of CD4+ T Cells While Upregulating Tregs

Figure 9

Effects of different commercial preparations of IVIg which include GamaSTAN™ S/D (15–18 gm%, lot 26NHCVI; Telacris Biotherapeutics Inc) at dilutions 1/10 (conc. 15 mg/ml) and 1/80 (conc. 1.2 mg/ml), Octagam® (6 gm%, lot A913A8431; Octapharma Pharmazeutika) at dilutions 1/20 (conc. 3 mg/ml) and 1/80 (conc. 0.75 mg/ml), and Gamunex®-C (10 gm%, lots 26NKLG1 and 26NKLK1, Telacris) at dilutions 1/10 (conc. 10 mg/ml) and 1/80 (conc. 8 mg/ml) (a) and mAb TFL-007a (at dilution 1/10, conc. 62.7 μg/ml; 1/80, conc. 7.84 μg/ml) (b) on PHA-untreated cells were compared with the effect of medium alone on the proliferation of Treg cells, defined as CD4+/CD25+/Foxp³+. Note that IVIg preparations used in this study failed to upregulate Tregs in contrast to TFL-007a which significantly upregulates Treg cells. p² (two-tailed value) (M. Taniguchi and M. H. Ravindranath, manuscript in preparation).

(a)

(b)