HLA Class Ia and Ib Polyreactive Anti-HLA-E IgG2a Monoclonal Antibodies (TFL-006 and TFL-007) Suppress Anti-HLA IgG Production by CD19+ B Cells and Proliferation of CD4+ T Cells While Upregulating Tregs

Table 2

The blastogenesis of PHA-activated CD4+ T cells was inhibited by anti-HLA-E mAbs TFL-006 and TFL-007 but not by negative control mAbs or by HLA-Ia mAbs or by nonpolyreactive HLA-E mAb TFL-037. Protein concentrations of culture supernatants are not given as they contain several exogenous and endogenous proteins. p² refers to two-tailed value (for further details see [47]).

Negative control mAbs	Mean	SD	p^2
No PHA	264	14
With PHA	969	117	0.0005
PHA + Hu IgG1 control	1758	84	NS
PHA + mAb 2124 (anti-HLA-A11/A43)	1758	84	NS
PHA + mAb 9123 (anti-HLA-Ia+)	1435	276	NS
Experimental anti-HLA-E mAbs
PHA + mAb TFL-006 (1/100) 8.870 mg/ml	502	184	0.02
PHA + mAb TFL-007 (1/100) 6.270 mg/ml	640	137	0.03
PHA + mAb TFL-037 (1/100) 6.000 mg/ml	911	54	NS

p² refers to 2-tailed value. The value for “with PHA” is against “no PHA”;the other values are against “PHA only.”