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Journal of Immunology Research
Volume 2017, Article ID 3908289, 19 pages
Research Article

A Novel System for the Quantification of the ADCC Activity of Therapeutic Antibodies

Biomonitor SAS, Villejuif Bio Park, 1 Mail du Professeur Georges Mathé, 94800 Villejuif, France

Correspondence should be addressed to Michael G. Tovey; rf.nomoib@tm

Received 23 May 2017; Revised 19 July 2017; Accepted 1 August 2017; Published 27 September 2017

Academic Editor: Douglas C. Hooper

Copyright © 2017 Christophe Lallemand et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Novel ADCC effector cells expressing the V-variant or F-variant of FcγRIIIa (CD16a) and firefly luciferase under the control of a chimeric promoter incorporating recognition sequences for the principal transcription factors involved in FcγRIIIa signal transduction, together with novel target cells overexpressing a constant high level of the specific antigen recognized by rituximab, trastuzumab, cetuximab, infliximab, adalimumab, or etanercept, confer improved sensitivity, specificity, and dynamic range in an ADCC assay relative to effector cells expressing a NFAT-regulated reporter gene and wild-type target cells. The effector cells also contain a normalization gene rendering ADCC assays independent of cell number or serum matrix effects. The novel effector and target cells in a frozen thaw-and-use format exhibit low vial-to-vial and lot-to-lot variation in their performance characteristics reflected by CVs of 10% or less. Homologous control target cells in which the specific target gene has been invalidated by genome editing providing an ideal control and a means of correcting for nonspecific effects were observed with certain samples of human serum. The novel effector cells and target cells expressing noncleavable membrane-bound TNFα have been used to quantify ADCC activity in serum from patients with Crohn’s disease treated with infliximab and to relate ADCC activity to drug levels.