Novel Chemokine-Based Immunotoxins for Potent and Selective Targeting of Cytomegalovirus Infected Cells

Table 1

Homologous binding experiments testing the binding affinity of the FTPs.

	US28/^125I-CX3CL1		CX3CR1/^125I-CX3CL1		Binding selectivity
	log IC50   SD	IC50 [nM]	log IC50  ± SD	IC50 [nM]	CX3CR1 versus US28 (CX3CL1)
CX3CL1-FTP	−8.9 ± 0.26	1.3	−7.3 ± 0.45	50	40
K14E-FTP	−7.2 ± 0.31	63	−6.2 ± 0.63	631	10
F49A-FTP	−7.5 ± 0.59	32	−5.6 ± 0.81	2512	79
F49L-FTP	−8.2 ± 0.95	6.3	−5.5 ± 0.36	3162	501
F49E-FTP	−6.5 ± 0.07	316	−5.3 ± 0.40	5012	16
F49A-FTP 2	−7.4 ± 0.49	40	−6.9 ± 0.50	126	3
F49A-FTP 3	−7.3 ± 0.64	50	>5,0		—
F49A-FTP 4	−7.6 ± 0.24	25	−6.5 ± 0.21	316	13
F49A-FTP 7	−7.3 ± 0.38	50	−6.6 ± 0.20	251	5

IC₅₀ values of the FTPs bound to CX₃CR1 have been estimated from a partial curve.
IC50 value estimated from 3-4 experiments.