Journal of Immunology Research / 2017 / Article / Fig 2

Research Article

Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils

Figure 2

Effect of STK and Syk inhibition on inflammatory cytokine generation in human neutrophils. Cells were pretreated for 30 min in the absence or presence of STK inhibitors (10 μM of PP1, PP2, or Srcl1) or of a Syk inhibitor (10 μM piceatannol), prior to stimulation for 6 h with 1 μg/ml LPS, 100 U/ml TNFα, 30 nM fMLP, or 1 nM GM-CSF. Culture supernatants were analyzed in ELISA. Results are expressed as mean ± SEM from at least 3 independent experiments, each performed in duplicate. §, versus unstimulated cells (“ctrl”); versus matched condition without inhibitor; versus matched condition without inhibitor.