Probiotic Lactobacillus Strains Stimulate the Inflammatory Response and Activate Human Macrophages

Figure 1

ROS production by human macrophages in response to probiotic heat-killed lactobacillus was measured by luminol-enhanced chemiluminescence. (a) Mixtures were prepared using 1 ml of Hank’s solution, containing luminol 0.8 × 10⁻⁴ M, 4 U HRP, and lactobacillus bacteria at different MOI (1 : 1 and 1 : 500; bacteria : macrophages). The kinetics of the oxidative response induced in human macrophages by lactobacillus was monitored (b). The values shown represent four experiments that were performed in duplicate. Asterisks indicate the level of statistical significance between conditions. Significant difference: .

(a)

(b)