Research Article
Secreted Interferon-Inducible Factors Restrict Hepatitis B and C Virus Entry In Vitro
Figure 5
IFN-α induced products inhibit HBV binding to the cells. (a) Heparin columns were washed with PBS and then applied with IFN-α treated differentiated HepaRG cell culture medium. Then columns were washed with PBS. Differentiated HepaRG cells were incubated with elution or flow-through for 24 hours followed by 4 hours of HBV incubation at 4°C. (b) Cells were lysed and cellular HBV-DNA were evaluated by qPCR. Data are means ± s.d. , , and by Student’s unpaired two-tailed -test.
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