Synergistic Antitumour Properties of viscumTT in Alveolar Rhabdomyosarcoma

Figure 4

viscumTT alters the expression of antiapoptotic proteins. RMS-13 cells were treated with viscum, TT, and viscumTT in increasing concentrations for 24 h. Afterwards, cells were lysed and whole proteins lysates were analysed by Western blot for altered expression of X-linked inhibitor of apoptosis protein (XIAP), baculoviral IAP repeat containing 5 (BIRC5), myeloid cell leukemia 1 (MCL1), B-cell lymphoma 2 (BCL2), and BCL2 like 1 (BCL2L1). β-actin served as a loading control. Mistletoe lectin (ML) and oleanolic acid (OA) concentrations were used as marker substances for viscum and TT, respectively.