RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells

Figure 1

Effect of B18 on type I IFN response. L929 cells were incubated with recombinant B18 protein (B18), with mouse IFNα (IFN), or with recombinant B18 and then mouse IFNα (B18 + IFN), and analyzed by RNA-seq. (a) Corresponding plots representing expression of all cellular genes. value (log 2 fold change) from each transcript between untreated and indicated sample cells is plotted against (overall average log expression level) of each untreated and indicated pair. Red dots indicate SDEGs when compared to untreated cells after differential expression analysis. (b) Heatmap of SDEGs identified after IFN treatment. The heatmap displays the fold change expression (log 2) in the indicated samples relative to results for untreated cells. The colour scale is shown at the bottom of the heatmap. (c) Enriched canonical pathways after IPA analysis with SDEGs identified after IFN induction.

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