RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells

Figure 6

Confirmation of RNA-seq data by RT-qPCR during VACV infection. Gene expression for the indicated genes was assessed by RT-qPCR from VACV- or VACVΔB18-infected L929 cells at 4 hpi and 9 hpi. IFN was added to infected cells at 5 hpi where indicated. Expression levels of WR092 and WR127 VACV genes were also determined to monitor the progress of infection and relativized to VACV 4 hpi expression values. Mean from 3 biological replicates ± SEM and significant differences are displayed.