DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture

Figure 2

Overexpressed DEPTOR does not inhibit lipogenesis and inflammation. (a) A schematic model showed the signaling of mTORC1-controlled lipogenesis. ACL, ACC1, FASN, and SCD1 were downstream enzymes in fatty acid synthesis. (b) Real-time RCR results showed that mRNA levels of lipogenesis genes, including Acl, Acc1, Fasn, and Scd1, were not reduced in DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. N.S.: no statistical significance. (c) Real-time RCR results showed that mRNA levels of inflammation genes, including IL1-β, IL-6, and TNF-α, were not reduced in DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. N.S.: no statistical significance.

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