DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture

Figure 3

AKT inhibitors downregulate lipogenesis and inflammation in DEPTOR overexpressed cells. (a) Western blots and quantifications showed that mTORC1 activity, indicated by phosphorylations of p70S6K and 4EBP1, was downregulated by myc-DEPTOR overexpression and MK-2206 (1 μM for 4 hours, same as follows) conditions. However, mTORC2 activity (indicated by pAKT/AKT) was blocked by MK-2206 treatment in lymphocytes of PBMC culture. Results are averages of three independent experiments. Data represent mean ± SEM. Data represent mean ± SEM. and . (b) Real-time RCR results showed that mRNA levels of lipogenesis genes, including Acl, Acc1, Fasn, and Scd1, were dramatically reduced in MK-2206 treated DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. Data represent mean ± SEM. , , and . (c) Real-time RCR results showed that mRNA levels of inflammation genes, including IL1-β, IL-6, and TNF-α, were dramatically reduced in MK-2206 treated DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. and . (d) ELISA results showed that protein levels of TNF-α were decreased in MK-2206 treated DEPTOR overexpressed cells. Results are averages of three independent experiments. Data represent mean ± SEM. .

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