DEPTOR-mTOR Signaling Is Critical for Lipid Metabolism and Inflammation Homeostasis of Lymphocytes in Human PBMC Culture

Figure 4

DEPTOR knockdown increases mTORC1 activity, lipogenesis, and inflammation in lymphocytes of PBMC culture. (a) Western blots showed that mTORC1 activity, indicated by phosphorylations of p70S6K and 4EBP1, was upregulated by DEPTOR shRNA in lymphocytes of PBMC culture. Noted that pAKT was slightly downregulated by DEPTOR knockdown. (b) Real-time RCR results showed that mRNA levels of lipogenesis genes, including Acl, Acc1, Fasn, and Scd1, were increased in DEPTOR knockdown cells. Results are averages of three independent experiments. Data represent mean ± SEM. , , and . (c) Real-time RCR results showed that mRNA levels of inflammation genes, including IL1-β, IL-6, and TNF-α, were increased in DEPTOR knockdown cells. Results are averages of three independent experiments. Data represent mean ± SEM. , , and . (d) ELISA results showed that protein level of TNF-α was increased in DEPTOR knockdown cells. Results are averages of three independent experiments. Data represent mean ± SEM. .

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