Adenosine Triphosphate Promotes Allergen-Induced Airway Inflammation and Th17 Cell Polarization in Neutrophilic Asthma

Figure 1

(a) Protocols for the establishment of the OVA and LPS-induced murine model of neutrophilic asthma and experimental interventions. Mice were intranasally sensitized with OVA plus LPS on days 0, 1, 2, and 7 and then challenged with OVA on days 14, 15, 21, and 22. Suramin or apyrase was administered intratracheally at 30 min before each OVA challenge. One day after the final challenge, the mice were sacrificed for analyses. (b) Protocols for establishment of the murine model of neutrophilic asthma and experimental interventions in DO11.10 mice. DO11.10 mice were challenged with an aerosolized solution of OVA on days 0, 1, and 2, and suramin was administered intratracheally at 30 min before each OVA challenge. One day after the final challenge, the mice were sacrificed for analyses.

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