Modulation of Tumor-Associated Macrophages (TAM) Phenotype by Platelet-Activating Factor (PAF) Receptor

Figure 2

Inflammatory infiltrate in B16F10 melanoma and TC-1 tumors. Single cell suspensions of TC-1 (a) or B16F10 (b) tumors from WT and PAFR KO animals injected 15 days earlier were analyzed by flow cytometry. Cells were labeled with anti-CD45 antibody and 50,000 events were acquired per sample, using a FACSCanto cytometer. In the left panel: dot plots depicting SSC-A X CD45 expression (panleukocyte marker) of tumors from WT and PAFR KO animals. These plots were obtained by previously gating out debris and doublets. The gates indicate the CD45⁺ populations. In the right panels, we show the average percentage of CD45⁺ cells in each experimental group. Data were obtained from 3 independent experiments with 4 animals per experimental group. indicates .

(a)

(b)