Modulation of Tumor-Associated Macrophages (TAM) Phenotype by Platelet-Activating Factor (PAF) Receptor

Figure 3

Characterization of lymphoid tumor infiltrate. Total single cell suspensions of B16F10 (a) or TC-1 (b) tumor from WT and PAFR KO animals were analyzed by flow cytometry (50,000 events). The left panels show a representative analysis of the lymphocyte (CD4⁺ and CD8⁺) populations. The CD4 and CD8 populations were analyzed within the CD45⁺CD11b⁻ gate, after gating out debris and doublets. The panels to the right show the quantification of these experiments, represented as the average percentage of cells expressing CD4 or CD8, within the CD45⁺CD11b⁻ population. Data were obtained from 3 independent experiments with 4 animals per experimental group. indicates .

(a)

(b)