Modulation of Tumor-Associated Macrophages (TAM) Phenotype by Platelet-Activating Factor (PAF) Receptor

Figure 4

Characterization of myeloid tumor infiltrate. Total single cell suspensions of B16F10 (a) or TC-1 (b) tumors from WT and PAFR KO animals were labeled with antibodies against the indicated markers plus anti-CD45 and anti-CD11b and analyzed in a FACSCanto, where 50,000 events were acquired. In the left panels, we show a representative analysis of the myeloid populations from 3 independent experiments with 4 animals per group. The cells were analyzed within the CD45⁺CD11b⁺ gate, after exclusion of debris and doublets. In the right panel, we show the mean percentage of the frequency of Gr1⁺ and F4/80⁺ cells within the CD45⁺CD11b⁺ population. .

(a)

(b)