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Journal of Immunology Research
Volume 2017, Article ID 5689709, 17 pages
Research Article

Immunization with Bivalent Flagellin Protects Mice against Fatal Pseudomonas aeruginosa Pneumonia

1Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
2Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3Burn Research Center, Hazrat Fatima Hospital, Iran University of Medical Sciences, Tehran, Iran
4Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
5Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Correspondence should be addressed to Abbas Ali Imani Fooladi; moc.liamg@a.idaluofinami

Received 6 January 2017; Revised 25 August 2017; Accepted 10 September 2017; Published 19 October 2017

Academic Editor: Peirong Jiao

Copyright © 2017 Bahador Behrouz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pseudomonas aeruginosa lung infections present a major challenge to healthcare systems worldwide because they are commonly associated with high morbidity and mortality. Here, we demonstrate the protective efficacy of type a and b flagellins (bivalent flagellin) against acute fatal pneumonia in mice. Mice immunized intranasally with a bivalent flagellin vaccine were challenged by different flagellated strains of P. aeruginosa in an acute pneumonia model. Besides the protective effect of the vaccine, we further measured the host innate and cellular immunity responses. The immunized mice in our study were protected against both strains. Remarkably, active immunization with type a or b flagellin significantly improved survival of mice against heterologous strain compared to flagellin a or b antisera. We also showed that after an intranasal challenge by P. aeruginosa strain, neutrophils are recruited to the airways of vaccinated mice, and that the bivalent flagellin vaccine was proved to be protective by the generated CD4+IL-17+ Th17 cells. In conclusion, bivalent flagellin vaccine can confer protection against different strains of P. aeruginosa in an acute pneumonia mouse model by eliciting effective cellular and humoral immune responses, including increased IL-17 production and improved opsonophagocytic killing.