Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)

Figure 8

Designation of light chain reactive monoclonal antibody specificity. Mouse anti-ferret Ig mAb (4-B10 and 8-H9) were used in combination with anti-CD79β and DyLight 650 conjugated Protein L to assign mAb reactivity with kappa light chain expressing B cells. (a) Representative surface staining of ferret PBMC with anti-CD79β and Protein L. Kappa light chain expressing B cells were defined as CD79β⁺ cells that stained intensely with DyLight 650 conjugated Protein L and were gated as shown in (a). The mean frequency of total B cells ± SD that stained intensely with Protein L is indicated. (b) Representative costaining of kappa light chain expressing B cells with 4-B10 or 8-H9. The mean frequency ± SD of total kappa B cells exhibiting costaining with 4-B10 or 8-H9 is indicated. Data in (a) and (b) are combined from two independent experiments (). (c) Distribution of kappa or lambda light chain expression by total B cells (CD79β⁺) was determined on the basis of costaining with 4-10 or 8-H9. Data in (c) are combined from three independent experiments (). Statistical significance was determined using a paired Student’s -test. .