Research Article

Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)

Table 1

Summary of MαF-Ig mAb.

mAbIg subclassSpecificity

4-B10IgG1IGHV4-1IGKV6-13Light chain
4-D11IgG1IGHV2-3IGKV16-104Light chain
4-EIgG1IGHV1-20 or IGHV1-37IGKV3-12Light chain
6-B5IgG1IGHV5-6IGKV19-93Ferret Ig
6-B7IgG1IGHV5-6Heavy chain
6-H5IgG1IGHV5-17IGKV5-48Light chain
8-H9IgG1IGHV14-3IGKV6-32Light chain
11-E3IgG1IGHV1S81IGKV6-17Heavy chain

a: identified using IMGT V-Quest (see [21]). b: unable to unambiguously assign IGHV. c: determined by western blot. d: determined by flow cytometry. e: not determined. f: negative for intracellular Igκ by flow cytometry. g: determined by competitive ELISA.