pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity

Figure 4

Effect of previous vaccination with pVAXhsp65 on peripheral immune response. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and then submitted to EAE induction. Cytokine production was assessed 30 days after EAE induction. IFN-γ (a), IL-6 (b), IL-17 (c), and IL-10 (d) production were assayed in spleen cell cultures restimulated in vitro with or rhsp65. The percentage of CD4+CD25+Foxp3+ T cells was evaluated in the total number of spleen cells 30 days after EAE induction (e). Data were presented by mean ± SE of 6 mice and are representative of two independent experiments. represents the difference between DNA injected groups and control group with EAE. .

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