Journal of Immunology Research

Research Article

Vaccinomics Approach for Designing Potential Peptide Vaccine by Targeting Shigella spp. Serine Protease Autotransporter Subfamily Protein SigA

Table 3

The potential CD4⁺ T-cell epitopes along with their interacting MHC class II alleles with affinity (IC50) < 100 nM and respective predivac scores.


Epitope	Interacting MHC-II allele (IC₅₀) on the nM scale	Number of alleles	Predivac score (binding core)

VTARAGLGYQFDLFA	HLA-DRB1∗04:01, HLA-DRB1∗09:01, HLA-DRB1∗04:05, HLA-DRB3∗01:01, HLA-DQA1∗05:01	5	68.76 (LGYQFDLFA)
NSGFHTVTVNTLDAT	HLA-DRB1∗01:01 (53), HLA-DRB1∗01:21 (17.87), HLA-DRB1∗01:17 (77.94), HLA-DRB1∗01:16 (98.93), HLA-DRB1∗01:13 (61.53), HLA-DRB1∗01:29 (83.08), HLA-DRB1∗01:24 (62.89), HLA-DRB1∗01:10 (28.18), HLA-DRB1∗01:11 (45.39), HLA-DRB1∗01:19 (39.99), HLA-DRB1∗01:12 (39.99), HLA-DRB1∗01:31 (39.99), HLA-DRB1∗01:32 (39.99), HLA-DRB1∗01:08 (39.99), HLA-DRB1∗01:05 (39.99), HLA-DRB1∗01:07 (39.99), HLA-DRB1∗01:27 (39.99), HLA-DRB1∗01:25 (39.99), HLA-DRB1∗01:22 (39.99), HLA-DRB1∗01:28 (39.99), HLA-DRB1∗01:14 (47.44), HLA-DRB1∗01:09 (37.12), HLA-DRB1∗01:15 (71.58), HLA-DRB1∗01:18 (44.25), HLA-DRB1∗01:06 (35.78), HLA-DRB1∗01:26 (38.4), HLA-DRB1∗01:20 (41.15), HLA-DRB1∗01:23 (48.69), HLA-DRB1∗01:04 (48.54), HLA-DRB1∗07:06 (65.25), HLA-DRB1∗07:05 (65.06), HLA-DRB5∗02:05 (46.61)	32	71.56 (FHTVTVNTL)
KAIELAGTLTLTGTP	HLA-DRB1∗01:01 (82), HLA-DRB1∗01:21 (23.85), HLA-DRB1∗01:17 (98.16), HLA-DRB1∗01:24 (58.9), HLA-DRB1∗01:10 (29.05), HLA-DRB1∗01:11 (49.8), HLA-DRB1∗01:19 (44.72), HLA-DRB1∗01:12 (44.72), HLA-DRB1∗01:31 (44.72), HLA-DRB1∗01:32 (44.72), HLA-DRB1∗01:08 (44.72), HLA-DRB1∗01:05 (44.72), HLA-DRB1∗01:07 (44.72), HLA-DRB1∗01:27 (44.72), HLA-DRB1∗01:25 (44.72), HLA-DRB1∗01:22 (44.72), HLA-DRB1∗01:28 (44.72), HLA-DRB1∗01:03 (91.98), HLA-DRB1∗01:14 (54.39), HLA-DRB1∗01:09 (31.87), HLA-DRB1∗01:15 (59.62), HLA-DRB1∗01:18 (40.48), HLA-DRB1∗01:06 (14.26), HLA-DRB1∗01:26 (18.43), HLA-DRB1∗01:20 (17.57), HLA-DRB1∗01:23 (24.27), HLA-DRB1∗01:04 (18.63), HLA-DRB1∗01:01 (82), HLA-DRB5∗02:05 (70.95)	29	71.70 (IELAGTLTL)
NNQIASFSQPDWESR		0	55.02 (FSQPDWESR)
VTARAGLGYQFDLFA		0	68.76 (LGYQFDLFA)
AQNYAWVNGNIKSDK	HLA-DRB5∗01:01 (62), HLA-DRB5∗02:04 (93.07), HLA-DRB5∗02:05 (24.55)	3	78.78 (YAWVNGNIK)
AAKSYMSGNYKAFLT	HLA-DRB1∗08:05 (65.65), HLA-DRB1∗12:03 (94.66), HLA-DRB1∗01:09 (57.66), HLA-DRB1∗01:10 (98.95), HLA-DRB1∗01:19 (99.23), HLA-DRB1∗01:12 (99.23), HLA-DRB1∗01:31 (99.23), HLA-DRB1∗01:32 (99.23), HLA-DRB1∗01:08 (99.23), HLA-DRB1∗01:05 (99.23), HLA-DRB1∗01:07 (99.23), HLA-DRB1∗01:27 (99.23), HLA-DRB1∗01:25 (99.23), HLA-DRB1∗01:22 (99.23), HLA-DRB1∗01:28 (99.23), HLA-DRB1∗01:21 (73.89), HLA-DRB1∗01:06 (52.24), HLA-DRB1∗01:23 (86.01), HLA-DRB1∗01:04 (87.14), HLA-DRB1∗01:26 (83.15), HLA-DRB1∗01:20 (85.84), HLA-DRB5∗02:05 (12.01), HLA-DRB5∗02:02 (67.2), HLA-DRB5∗02:04 (62.02)	24	60.30 (KSYMSGNYK)
SYMSGNYKAFLTEVN	HLA-DRB1∗01:21 (56.34), HLA-DRB1∗01:09 (89.52), HLA-DRB1∗01:10 (81.52), HLA-DRB1∗01:26 (98.98), HLA-DRB1∗01:06 (88.25), HLA-DRB1∗04:05 (38), HLA-DRB5∗02:05 (41.74)	7	69.98 (YKAFLTEVN)
ASMYNTLWRVNGQSA	HLA-DRB1∗08:05 (35.19), HLA-DRB1∗13:01 (49.45), HLA-DRB1∗12:05 (52.01), HLA-DRB1∗12:02 (60.35), HLA-DRB1∗12:03 (50.74), HLA-DRB1∗01:23 (62.08), HLA-DRB1∗01:26 (66.37), HLA-DRB1∗01:20 (74.19), HLA-DRB1∗01:06 (73.79), HLA-DRB5∗02:05 (44.37), HLA-DRB1∗11:01 (70)	11	82.45 (LWRVNGQSA)