Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity

Figure 2

Levels of the total APN and HMW APN in plasma during the different stages of HFRS. Changes in the levels of (a) total APN and (b) HMW APN in the plasma of the same individuals from different disease severity groups are shown. (c) Under nonreducing and non-heat-denaturing conditions, a comparison of the plasma APN levels in the acute phase (the febrile, hypotensive, or oliguric stages), the convalescent phase (conv; the diuretic stage or convalescent stage), and 6 months after discharge in the same patients with HFRS and in normal controls is analysed by western blot (representative results of 3 patients with HFRS and 2 healthy subjects). Trimeric low molecular weight (LMW, 68 kDa), albumin-bound LMW (ALB-LMW, 100 kDa), hexameric middle molecular weight (MMW, 150 kDa), and HMW (>250 kDa) forms of APN are marked by black arrows. Plasma LMW plus ALB-LMW APN, MMW APN, and HMW APN levels in patients with HFRS were all elevated in the acute phase and became higher in the convalescent phase, whereas the levels of all different forms of plasma APN recovered to normal levels when measured 6 months after discharge.

(a)

(b)

(c)