Th1/Th2 Balance and Th17/Treg-Mediated Immunity in relation to Murine Resistance to Dextran Sulfate-Induced Colitis

Figure 3

Lymphocyte subsets of LPL and MLN in DSS-challenged BALB/c and C57BL/6 mice as measured by FACS. Data indicate the mean ± SD of three animals, representative of two independent experiments. Statistical significant differences were evaluated by Student’s t-test. ; . The mononuclear cells were harvested from LP and MLN 6 days after DSS induction and strained with various combinations of mAbs (neutrophil: CD11b⁺Gr-1^high; monocyte: CD11b⁺F4/80⁺; activated T cells: CD4⁺TCRαβ⁺CD69⁺). In all assays, the physical (day 0) cell number of immune cell subtypes is 200,000/sample. (a), (b) The isotype stainings were performed using a different flow cytometer and thus cannot be directly compared and hence are not shown.

(a)

(b)