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Figure 6: Inhibition of Th17 associated factors in inflammatory skin lesions and serum by EKC. (a) Mice were sacrificed, and protein was extracted from the back skin of each mouse. Cytokine levels were measured by ELISA. Values represent the mean ± SD. (b) Total mRNA was extracted from the skin using TRIzol, and the levels of IL-23p19 and IL-12p40 were measured by real-time PCR. Values represent the median. (c) The skin samples were prepared by homogenization with T-PER protein extraction buffer. p-IκB-α expression levels were determined by Western blot analysis. The intensity of β-actin staining in each sample was used as a loading control. (d) The concentration of IL-17A and IL-12p40 collected in serum on day 28 was determined by ELISA. Values represent the mean ± SD. (e) Concentration of IgG1 in the collected serum was measured by ELISA. Values represent the mean ± SD. ; ; and compared with the IMQ + water group.