Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice

<div>Cellular localization of RT without signal of secretion (RT1.14opt-in) and with a signal peptide (RT1.14oil) in HeLa cells. HeLa cells were transfected with the plasmids pVaxRT1.14opt-in (a, c, e) and pVaxRT1.14oil (b, d, f), grown on slides for 48 hours, fixed with methanol : acetone (1 : 1 <i>v</i>/<i>v</i>) (a, b, e, f) or 2% paraformaldehyde (c, d), and stained first with polyclonal anti-RT and with secondary FITC-conjugated anti-rabbit antibodies. For localization of RT in relation to ER, HeLa cells were treated with TRITC-conjugated antibodies to an ER marker calreticulin (e, f), and the overlay of FITC and TRITC signals is shown.</div>

Journal of Immunology Research

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Figure 3

Figure 3: Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice 

Figure 3 | Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice 