Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice

Figure 9

Kinetics of in vivo bioluminescence from the sites of coadministration of RT genes and luciferase reporter genes. In vivo monitoring of luciferase activity on days 1, 3, 6, 9, 15, 22 after the administration of plasmids encoding RT1.14opt-in and RT1.14oil, or empty vector pVax1, each mixed with pVaxLuc encoding firefly luciferase (1 : 1 w/w). One curve represents bioluminescent emission from eight mice with two immunization sites in each, assessed in two independent immunization runs. (Mann–Whitney test).