Research Article
Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice
Table 1
Expression, secretion, and residual enzymatic activity of inactivated forms of HIV reverse transcriptase RT1.14opt-in and RT1.14oil in transiently transfected HeLa cells. RT content was determined by Western blotting using protein calibration curves with signal quantification by ImageJ (dubbed WB, in μg/105 cells) and by quantification of enzymatic activity against standard RT preparation using Lenti RT activity test (Cavidi, Sweden) (dubbed EA, in pg/105 cells). RT content in cell lysates is dubbed “in cells” and in cell culture fluids “secreted.” Specific activity was measured as a ratio of protein content evaluated using the enzymatic activity assay to RT content determined by Western blotting.
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