Mahuang Fuzi Xixin Decoction Attenuates Th1 and Th2 Responses in the Treatment of Ovalbumin-Induced Allergic Inflammation in a Rat Model of Allergic Rhinitis

Figure 5

Effect of MFXD on the percentages of CD3⁺CD4⁺IFN-γ⁺ Th1 and CD3⁺CD4⁺IL-4⁺ Th2 cells in peripheral blood mononuclear cells (PBMCs) of AR rats. PBMCs of rats were separated from anticoagulant blood sample, stained with fluorescently labeled anti-rat antibodies, and analyzed by flow cytometry. (a) Representative flow cytometry dot plots for each groups, and the plots in the upper right quadrant indicate the percentage of CD3⁺CD4⁺IFN-γ⁺ Th1 and CD3⁺CD4⁺IL-4⁺ Th2 cells among PBMCs. Percentages of (b) CD3⁺CD4⁺IFN-γ⁺ Th1 cells and (c) CD3⁺CD4⁺IL-4⁺ Th2 cells in each groups. (d) Ratios of CD3⁺CD4⁺IFN-γ⁺ Th1 and CD3⁺CD4⁺IL-4⁺ Th2 cells were calculated. Data are expressed as mean ± SD; rats; versus model.

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