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Journal of Immunology Research
Volume 2017 (2017), Article ID 8903982, 7 pages
https://doi.org/10.1155/2017/8903982
Research Article

Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae

1Department of Pediatrics, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38103, USA
2Children’s Foundation Research Institute, Le Bonheur Children’s Hospital, Memphis, TN 38103, USA
3Department of Physiology, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38163, USA
4Department of Pharmacology, University of Tennessee Health Science Center (UTHSC), Memphis, TN 38163, USA

Correspondence should be addressed to D. Betty Lew; ude.cshtu@weld

Received 10 February 2017; Revised 5 May 2017; Accepted 30 May 2017; Published 9 July 2017

Academic Editor: Elizabeth H. Kemp

Copyright © 2017 Christie F. Michael et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from S. cerevisiae (SC-MN) on airway epithelial wound repair, in vitro. The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.