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Journal of Immunology Research
Volume 2017, Article ID 9152960, 12 pages
https://doi.org/10.1155/2017/9152960
Research Article

Neuroprotective Potential of Gentongping in Rat Model of Cervical Spondylotic Radiculopathy Targeting PPAR-γ Pathway

1College of Chemical and Biological Engineering, Yichun University, Yichun 336000, China
2Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China
3School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610000, China
4Beijing Yjheal Medical Research Center, Beijing 100100, China

Correspondence should be addressed to Jianfeng Yi; moc.qq@864561609 and Cheng Lu; moc.361@6180gnehc_vl

Received 2 May 2017; Revised 15 July 2017; Accepted 25 July 2017; Published 5 November 2017

Academic Editor: Qingdong Guan

Copyright © 2017 Wen Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cervical spondylotic radiculopathy (CSR) is the most general form of spinal degenerative disease and is characterized by pain and numbness of the neck and arm. Gentongping (GTP) granule, as a classical Chinese patent medicine, has been widely used in curing CSR, whereas the underlying mechanism remains unclear. Therefore, the aim of this study is to explore the pharmacological mechanisms of GTP on CSR. The rat model of CSR was induced by spinal cord injury (SCI). Our results showed that GTP could significantly alleviate spontaneous pain as well as ameliorate gait. The HE staining and Western blot results showed that GTP could increase the quantity of motoneuron and enhance the activation of peroxisome proliferator-activated receptor gamma (PPAR-γ) in the spinal cord tissues. Meanwhile, immunofluorescence staining analysis indicated that GTP could reduce the expression of TNF-α in the spinal cord tissues. Furthermore, the protein level of Bax was decreased whereas the protein levels of Bcl-2 and NF200 were increased after the GTP treatment. These findings demonstrated that GTP might modulate the PPAR-γ pathway by inhibiting the inflammatory response and apoptosis as well as by protecting the cytoskeletal integrity of the spinal cord, ultimately play a neuroprotective role in CSR.