Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus

Table 1

Conserved EBV-specific CD8 T cell epitopes from early antigens.

Epitope	Antigen gene	AN^1	HLA I restriction^2	Predicted HLA I profile	PPC%^3
RPPIFIRRL	EBNA3	P12977	B07, B08, B0702	B0702, B0801, B3501,; B5101, B5102, B5103,; B5301, B5401, C0102	57.84
SVRDRLARL	EBNA3	P12977	A0201	A0201, A0203, A0206,; A0214, B0702, B0801,; B1517	56.66
YVLDHLIVV	BRLF1	P03209	A0201, A02	A0201, A0202, A0203,; A0204, A0205, A0206,; A0209, A0214, B1517,; B5701	47.34
QPRAPIRPI	EBNA6	P03204	B0702	B0702, B3501, B5101,; B5102, B5103, B5301,; B5401, B5502	43.56
LPCVLWPVL	BZLF1	P03206	B0702	B0702, B3501, B5101,; B5102, B5103, B5301,; B5401	42.4
RVRAYTYSK	BRLF1	P03209	A0301, A03	A0301, A1101, A3101,; A3301, A6801	41.46
AYSSWMYSY	EBNA3	P12977	A30	A0101, B2701, C0702	36.38
VLKDAIKDL	EBNA1	P03211	A0203	A0203, A0204, A0205,; A0206, A0207, A0214,; B0801, C0304	33.72
QAKWRLQTL	EBNA3	P12977	B08	B0702, B0801, B1400,; C0102	32.48
RRIYDLIEL	EBNA6	P03204	B2705	B1400, B2702, B2703,; B2704, B2705, B2706,; B2709, C0702	30.42
RLRAEAQVK	EBNA3	P12977	A03, A0301	A0301, A1101, B1513	28.7
CYDHAQTHL	BMRF1	P03191	A2402	A0207, A2402, B3801	27.3
SENDRLRLL	BZLF1	P03206	B4002, B60	B4002, B4402	14.18
YRSGIIAVV	BMRF1	P03191	B3906, Cw6	A0202, A0203, A0204,; A0205, A0209, B1509,; B1510, B1516, B2709,; B3801, B39,011, B3909	12.82
ARYAYYLQF	DBP	P03227	B2705	B1400, B1517, B2701,; B2702, B2703, B2704,; B2705, B2706, B2709	7.56
VSFIEFVGW	EBNA3	P12977	B58	B5701, B5702	5.08

¹Antigen accession number from the UniProtKB database. ²Experimental restriction profile obtained from epitope databases. ³Average population protection coverage (PPC) of PPCs computed for 5 ethnic groups in the USA population (Black, Caucasian, Hispanic, North American natives, and Asians) using the relevant HLA I genetic frequencies [40]. The combination that reached the largest PPC (97.1%) included the CD8 T cell epitopes YVLDHLIVV, YRSGIIAVV, SVRDRLARL, RVRAYTYSK, LPCVLWPVL, and RRIYDLIEL.