Propyl Gallate Exerts an Antimigration Effect on Temozolomide-Treated Malignant Glioma Cells through Inhibition of ROS and the NF-κB Pathway

Figure 2

Evaluation of migration with TMZ, PG, or TMZ/PG treatment. (a, b) U87MG cells (1 × 10⁴) were plated in 24-well Millicell hanging cell culture inserts with an 8 μm pore size membrane for 24 h and then treated with TMZ (200 μM), PG (50 or 100 μM), and their combination for 48 h. (c, d) U87MG cells (1 × 10⁴) were plated in 24-well Millicell hanging cell culture inserts with an 8 μm pore size membrane for 24 h and then treated with TMZ (200 μM) or PG (50 or 100 μM) for 48 h or pretreated with PG (50 or 100 μM) for 6 h and then treated with (200 μM) for 48 h. (a, c) Transwell migration assay was carried out. The migrated cells were stained with crystal violet. The dye was eluted with 33% acetic acid, and crystal violet absorbance was measured at 570 nm using a microplate reader. The values are presented as mean ± standard deviation (–8) of individual experiments. Significant differences for the untreated group and TMZ group were , , , and ^##. (b, d) Random fields from each of the triplicate migration assays were counted using phase contrast microscopy (magnification 200x).

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