Research Article

Chinese Herbal Formula, Modified Danggui Buxue Tang, Attenuates Apoptosis of Hematopoietic Stem Cells in Immune-Mediated Aplastic Anemia Mouse Model

Figure 6

Expression and distribution of T-bet in bone marrow cells (BMCs) of mice with aplastic anemia (AA) after treatment. (a) Staining for T-bet (red) in BMCs was observed by confocal immunofluorescence microscopy. The bottom pictures were obtained in bright field. The stained cells (red) were quantitated with ImageJ. (b) Results are presented in the bar charts. The scale bar corresponds to 100 μm throughout. A, normal group, B, model group, C, group treated with cyclosporin A, and D, group treated with DGBX. (c) The expression of T-bet was evaluated in whole BMC lysates by Western blot analysis. (d) The results are presented in the bar chart. Data are presented as mean ± SD, . .
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