Adrenomedullin Regulates IL-1β Gene Expression in F4/80+ Macrophages during Synovial Inflammation

Figure 1

Characterization of macrophage cells in inflamed synovial tissue of C57BL/6J mice. Flow cytometric analysis of CD11c⁺F4/80⁺CD11b macrophage cells in the synovial tissue (ST) obtained from the control and dislocated side of a patellar surgical dislocation model generated in C57BL/6J mice (a–f). (a, b) Dot-plot analysis of F4/80⁺CD11b⁺ cells in ST of the control (a) and dislocated side (b) of mice; -axis, F4/80; -axis, CD11b. (c, d) Histogram analysis of CD11c⁺ cells in the gated regions in the dot-plots of ST cells isolated from the control (c) and dislocated side (d) of mice. Percentages of F4/80− and CD11b⁺ cells (e) and CD11c⁺ cells in the F4/80− and CD11b-positive gated regions in the ST of control and dislocated side (f) of mice (). (g) Real-time PCR analysis of CD11c expression in the ST obtained from the control and dislocated side of C57BL/6J mice (). Statistically significant difference between the control and dislocated side. All data are presented as the mean ± standard error ().

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