Regulation of Leukocyte Recruitment to the Spleen and Peritoneal Cavity during Pristane-Induced Inflammation

Figure 2

IL-6 deficiency decreased the recruitment of splenic macrophages in response to pristane treatment. (a, b) IL-6^−/− mice and WT mice were injected with 300 μL (8.3 × 10⁻⁴ mol) of pristane and sacrificed at three weeks thereafter. Single cell suspensions of the spleen (SP) and peritoneal cavity (PC) from pristane-injected WT and IL-6^−/− mice were prepared for cellularity analysis by flow cytometry. The total cell number and the number of each cell type were calculated, respectively. (c, d) The total number of cells or the number of each cell type in the PC was analyzed in the same way. The difference between WT and IL-6^−/− mice is shown. Data represent two independent experiments. , , and .

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