EGFR-Targeted Immunotoxin Exerts Antitumor Effects on Esophageal Cancers by Increasing ROS Accumulation and Inducing Apoptosis via Inhibition of the Nrf2-Keap1 Pathway

Figure 2

PT was rapidly internalized when bound to EGFR-overexpressed esophageal cancer cells. (a) Flow cytometric results, indicating cell surface EGFR expression in KYSE-150 cell lines. (b) Flow cytometric results, indicating cell surface EGFR expression in KYSE-450 cell lines. (c) Analysis of internalization rates of PT in KYSE-150 cells when binding to EGFR. (d) Analysis of internalization rates of PT in KYSE-450 cells when binding to EGFR. KYSE-150 or KYSE-450 cells were incubated with saturating level of PT (10 μg/mL) for 30 minutes at 4°C. Unbound antibody conjugates were removed by washing cells. Cells were then incubated at either 4°C or 37°C. At the indicated time points, samples were detected by flow cytometry. Points: mean of 3 independent determinations; bars: SD.

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