Conventional DCs from Male and Female Lupus-Prone B6.NZM Sle1/Sle2/Sle3 Mice Express an IFN Signature and Have a Higher Immunometabolism That Are Enhanced by Estrogen

Figure 9

Female and male TCSle cDCs have a higher immunometabolism. Bone marrow-derived cDCs from B6 and TCSle female and male mice were cultured in standard conditions. On day 7, cDCs were stimulated with CpG (10 μg/mL) or R848 (1 μg/mL). cDCs were harvested 6 hours post stimulation for qRT-PCR analysis. Pdk1 was normalized to the housekeeping gene cyclophilin (a). Standard female unstimulated B6 condition was set to 1 in each experiment. Supernatants were harvested at the 24-hour time point and analyzed using the Griess reaction to measure nitrites (b). Dotted lines at 18 and 40 represent female B6 levels after CpG and R848 stimulation. Mean + SE values are from 3 independent experiments, using one mouse of each strain and sex per experiment (a, b). Two-way ANOVA analysis with Tukey multiple comparisons was used to determine significant activation by CpG or R848 within each group of mice represented by brackets and below the graph. Two-way ANOVA analysis with Tukey multiple comparisons, used to compare differences between B6 and TCSle, is represented by brackets and above the bars. , , and .

(a)

(b)