Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3⁺ T Cell through Modulation of CD11b⁺ Cell Function

Figure 4

Foxp3⁺ regulatory T cells play an important role in protecting against the deleterious effects of IL-22 in aGVHD. D2B6F1 mice were injected intraperitoneally with PC61 (anti-CD25 monoclonal antibody; 250 g/mouse) or control IgG simultaneous with transfer of B6 cells. aGVHD was induced as previously described. After 14 days, mice were sacrificed, and donor cells and host cells were enumerated using flow cytometry. (a) Representative plots of donor and host cells 14 days after cell transfer. (b) Number of donor CD8⁺ and host B cells. (c) Relative engraftment percentage and total number of donor cells. Data are shown as the mean ± SEM from five independent experiments. .

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