Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3⁺ T Cell through Modulation of CD11b⁺ Cell Function

Figure 7

IL-22Ab regulated the phenotype and function of CD11b⁺ cells in aGVHD mice. (a) Relative expression of IL-22R1 mRNA in CD11b⁺ cells from aGVHD mice at different time points. The day 0 vehicle was arbitrarily assigned a value of 1, with the other values shown relative to this value. (b) Expression of TGF-β, IL-6, IL-18, IFN-γ, and IL-18 in CD11b⁺ cells from aGVHD mice treated with anti-IgG or IL-22Ab on days 2 and 9. (c) Relative expression of CD80, CD86, CD83, and MHC-II mRNA in CD11b⁺ cells from aGVHD mice treated with anti-IgG or IL-22Ab on days 2 and 9 examined using RT-PCR. The day 2 vehicle in the anti-IgG group was arbitrarily assigned a value of 1, with the other values shown relative to this value. (d) The expression of Stat1, P-Stat1, Stat3, and P-Stat3 in CD11b⁺ cells examined using Western blotting. (e) Levels of β-defensin and Reg3γ mRNA in CD11b⁺ cells examined using RT-PCR. The anti-IgG vehicle was arbitrarily assigned a value of 1, with the other values shown relative to this value. Data are shown as the mean ± SEM from four independent experiments. and .

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