Association of Cytotoxic T Lymphocyte Antigen-4 Gene Polymorphisms with Psoriasis Vulgaris: A Case-Control Study in Turkish Population

Table 2

Primers, conditions for digestion, products of digestion, and genotypes according to products of digestion.

SNP	Primers	Amplicon (bp)	RE	Temperature and duration of digestion	Products of digestion (bp) and genotypes
−318C>T	F: 5-AAATGAATTGGACTGGATGGT-3; R: 3-TTACGAGAAAGGAAGCCGTG-5	247	MseII	37°C, overnight	CC: 247; CT: 20, 95, 132, 247; TT: 20, 95, 132
+49A>G	F: 5-TTGCTCTACTTCCTGAAGACCTGAA-3; R: 3-AAAGTCTCACTCACCTTTGCAGAAG-5	166	BbvI	37°C, overnight	AA: 166; AG: 76, 90, 166; GG: 76, 90
CT60 A>G	F: 5-CAC CACTATTTGGGATATACC-3; R: 3-AGGTCTATATTTCAGGAAGGC-5	216	NcoI	37°C, overnight	AA: 20, 196; AG: 20, 196, 216; GG: 216