Acute Neuroinflammatory Response in the Substantia Nigra Pars Compacta of Rats after a Local Injection of Lipopolysaccharide

Figure 11

Time course of leucocyte infiltration. (a) Representative micrographs of the double immunofluorescence of CD45 and TH in the substantia nigra of untreated (Ut) control rats and rats at different times after LPS injection that were taken at 3.4 mm from the interaural midpoint on the dorsal-ventral axis of the rat brain atlas by Paxinos and Watson [50]. The numbers at the left side of micrographs indicate the time of evaluation. Immunofluorescence (IF) area density for TH (b) and CD45 (c) was determined using ImageJ software v.1.46r (National Institutes of Health, Bethesda, MD). The TH and CD45 values for the mock rats correspond to the quantification in Supplementary Figure 3. All values represent the mean ± SD ( rats for each time and for each experimental condition). when compared with the untreated control group of the respective immunostaining. when compared with the respective mock group. Repeated-measures two-way ANOVA and Bonferroni post hoc test.

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