Investigation of Outbreak-Specific Nonsynonymous Mutations on Ebolavirus GP in the Context of Known Immune Reactivity

Figure 1

Mapping of antibody responses with sequence identity along the entire GP protein. Immunome Browser epitope mapping of all antibody responses per residue of the reference GP sequence (blue) compared with plot of sequence identity for ebolavirus GP NCBI reference sequences (grey) and all EBOV variants (red). Shaded regions represent the two subunits of the glycoprotein, GP₁ aa33-501 (yellow) and GP₂ aa502-676 (green). Region 1-33 represents the signal peptide (unshaded). Sequence identity is plotted as the running average with a window of 9. Sequence identities shown above represent the average for the region wherein responses are present (average of raw sequence ID scores). RFscores here were converted to percentages. Abbreviations: signal peptide: SP; receptor-binding domain (54-201): RBD; glycan cap (201-309): GC; mucin-like domain (305-485): MLD; internal fusion loop (524-539): IFL; heptad repeats (554-595, 615-634): HR; transmembrane region (651-671): TM.