Journal of Immunology Research

Research Article

Investigation of Outbreak-Specific Nonsynonymous Mutations on Ebolavirus GP in the Context of Known Immune Reactivity

Table 1

Correspondence of epitope data with nonsynonymous mutations on EBOV GP.


NSM	Response	IEDB antibody data						Mutational analyses
NSM	Response	Epitope ID (IEDB)	Host	Positive	Negative	GP location	Functional	Previous	Outbreak

29	Nonepitope	40693, 187386	H	NA	R	SS	NA	R	K
31	Nonepitope	227630, 40693, 187386, 227534	H	NA	F	SS	NA	F	S
46	Epitope	61752	H, M (16F6)	S	NA	GP1	N	S	N
47	Epitope	61752, 1902944	H, M (16F6)	E	NA	GP1	Y	E	D
82	Nonepitope	57019, 187449, 227411	H	NA	A	GP1; RB	NA	A	V
107	Epitope	227450	H	N	NA	GP1; RB	N	N	D
202	Epitope	13781	H	P	NA	GP1	N	P	L
206	Epitope	13781	H	T	NA	GP1	N	T	M
212	Epitope	13781	H	G	NA	GP1	N	G	D
213	Epitope	13781	H	Y	NA	GP1	N	Y	H
214	Epitope	13781	H	Y	NA	GP1	N	Y	H
222	Epitope	187549, 50368	H	A	NA	GP1	N	A	V
230	Epitope	50368, 187549, 227170	H	T	NA	GP1	N	T	A
239	Epitope	227097	H	L	NA	GP1	N	L	S
262	Epitope	502927	H	T	NA	GP1	Y	T	A
272^{^}	Epitope	442032	M (MB-003/13C6)	K	NA	GP1	Y	K	N
283^{^}	Nonepitope	187578, 25693	N	NA	T	GP1	NA	T	A
286	Epitope	478550	NHP (mAb FVM09)	G	NA	GP1	Y	G	R
291	Nonepitope	187262, 25693	H	NA	W	GP1	NA	W	R
314	Epitope	28398, 227137, 227646, 227406	H	G	NA	GP1; M	N	E	K/D
315	Epitope	28398, 227137, 227646, 227406	H	A	NA	GP1; M	N	A	P
326	Epitope	227409, 52394, 187509, 227406	H	T	NA	GP1; M	N	L	P
330	Epitope	227409, 52394, 187509, 227099	H	P	NA	GP1; M	N	P	S
331	Epitope	227409, 52394, 187509, 227099	H	G	NA	GP1; M	N	G	R
336	Epitope	187509, 227099, 227422	H	T	NA	GP1; M	N	T/L	N/M
354	Epitope	27448, 227141, 187269, 227664	H	H	NA	GP1; M	N	T	I
359	Epitope	227141, 187269, 227664, 227225	H	E	NA	GP1; M	N	G	K
367	Nonepitope	227664, 227225, 5540, 187276, 227378, 64732	H; M	NA	T	GP1; M	NA	T	A
371	Nonepitope	64732; 227052	H; M	NA	I	GP1; M	NA	I	V
375	Nonepitope	64732, 227052, 66371, 227635	H; M	NA	P	GP1; M	NA	P	S
382	Epitope	227307	H	P	NA	GP1; M	N	P	T
389	Epitope	187300, 233197, 227248	H; M (6D8)	H	NA	GP1; M	Y	H	R
395^{^}	Epitope	227307, 187300, 233197, 227248, 65808, 227687, 442034	H; M (6D8)	K	NA	GP1; M	Y	K	R/G/E
397^{^}	Epitope	227307, 187300, 233197, 227248, 65808, 227687, 442034, 8777, 187296	H; M (6D8)	D	NA	GP1; M	Y	D	G
398	Epitope	227687, 442034, 187296	H; M (6D8)	I	NA	GP1; M	Y	I	T
405	Epitope	8777; 187296; 233152; 68320; 13837	H; M (13F6)	E	NA	GP1; M	Y	E	G
406^{^}	Epitope	13837, 442031, 68320, 65808, 227687, 8777, 187296, 233152	H; M (13F6)	Q	NA	GP1; M	Y	Q	R
407	Epitope	13837; 68320; 8777; 187296; 233152	M (13F6)	H	NA	GP1; M	Y	H	Y
410	Epitope	13837; 68320; 8777; 187296; 233152	M (13F6)	R	NA	GP1; M	Y	R	S
411	Epitope	442031	M (13F6)	T	NA	GP1; M	N	T	A
419	Nonepitope	52688, 227526, 227403, 227048	H	NA	D	GP1; M	NA	D	E
430	Nonepitope	227048, 49300, 227596, 227449	H	NA	P	GP1; M	NA	P	L
439	Nonepitope	227596; 1641; 227449; 227142; 227633	H	NA	K	GP1; M	NA	K	E
440	Nonepitope	1641, 227449, 227142, 227633	H	NA	S	GP1; M	NA	G	S
441	Nonepitope	227449, 227142, 227633, 63152	H	NA	T	GP1; M	NA	T	A
443	Nonepitope	227449, 227142, 227633, 63152	H	NA	F	GP1; M	NA	L	S
446	Nonepitope	227142; 227633; 63152; 187301	H	NA	P	GP1; M	NA	P	L
448	Nonepitope	227633, 63152, 187301	H	NA	T	GP1; M	NA	T	A
455	Nonepitope	63152, 187301, 227493, 227641	H	NA	H	GP1; M	NA	H	Y
462	Epitope	156605	M (ZMAPP/4G7)	N	NA	GP1; M	Y	N	Y
472	Epitope	187541	H	E	NA	GP1; M	N	E	G
479	Epitope	187541, 162327	H; M (12B5 1-1)	L	NA	GP1; M	Y	L	P
480	Epitope	162327; 187541	H; M (14G7)	G	NA	GP1; M	Y	G	D
485	Epitope	162327	M (14G7; 12B5 1-1)	T	NA	GP1; M	Y	T	A
486	Epitope	162327	M (14G7; 12B5 1-1)	I	NA	GP1	Y	I	T
499	Epitope	156605	M (ZMAPP/4G7)	T	T	GP1	Y	A	T
503	Epitope	549302	M (4G7)	A	NA	GP2	N	A	V
545	Epitope	549302	H	E	NA	GP2	N	E	D
637	Nonepitope	51081; 187484; 227088; 68004; 227315; 187302	H; M	NA	D	GP2	NA	D	G

NSM: residue site of nonsynonymous mutation; Response: results from Immunome Browser mapping performed April 25, 2017; Epitope ID refers to individual, unique epitopes reported within the IEDB. More than one ID = more than one epitope (positive or negative); NSM and residue data summarized from [7, 9, 39, 38, 13, 14, 37]. All data are from GP or ssGP; Location: region on GP; Functional: designation for epitopes defined in the context of functional assay (virus neutralization/inhibition shown in vitro and/or protective in vivo). Nonepitope = tested and found to be negative (nonimmunogenic versus untested). Previously analyzed in [46]. RB: receptor-binding domain (54-201); M: mucin-like region (305-485). GP1 (33-501); GP2 (502-676); M: mouse Ab; H: human sera; ^{^}NSMs identified by [41] following failed MB-003 mAb cocktail trial in NHP.