Sirtuin1 Targeting Reverses Innate and Adaptive Immune Tolerance in Septic Mice

Figure 2

CD4+ T cell-associated cytokines are reprogrammed in a SIRT1-dependent manner. Cumulative scatterplot data of proinflammatory (a) CD4+TNFα+ T cells and (b) CD4+IFNγ+ T cells presented as frequency of CD4+ T cells in sham versus CLP versus CLP + EX-527. Cumulative scatterplot data of anti-inflammatory (c) CD4+TGFβ+ T cells and (d) CD4+IL10+ T cells presented as frequency of CD4+ T cells in sham versus CLP versus CLP + EX-527. (e) Representative ELISPOT data of IFNγ producing T cells ± restimulation from sham versus CLP versus CLP + EX-527 mice. (f) Cumulative results presented as spots counted per well from sham versus CLP versus CLP + EX-527, based on cells seeded at 3 × 10⁵ cells/well. Data are expressed as mean ± SEM. (a–d) mice/group; (e, f) mice/group. .

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