Natural Killer Cells from Malignant Pleural Effusion Are Endowed with a Decidual-Like Proangiogenic Polarization

Figure 8

Evaluation of NK cell-derived supernatants’ ability to induce morphogenesis on endothelial cells. Following 6 h exposition with NK cell-derived supernatants, tmPE-NK samples showed a significantly higher ability to induce the formation of capillary-like structures on HUVECs seeded on matrigel-precoated plates compared with samples derived from iPE-NK or ptPE-NK cells. Negative control (CTRL−) consisted in FBS-free Endothelial Basal Medium-2 (EBM, Lonza); positive controls (CTRL+) consisted in Endothelial Growth Medium-2 (EGM-2, Lonza), supplemented with bullet-kit (VEGF, FGF1, FGF2, insulin, hydrocortisone, and heparin; Lonza) containing 10% of FBS. Microphotographs (a) were taken at 10x magnification, and master segment lengths (b) were quantified by the Angiogenesis Analyzer ImageJ tool kit. Data are shown as mean ± SEM; and ( values are shown).

(a)

(b)