Analysis of ROR1 Protein Expression in Mice with Reconstituted Human Immune System Components

Figure 4

ROR1-expressing B cells were mostly immature B cells. (a) Flow cytometry staining of cells from the bone marrow, spleen, and blood of huNSG mice. Cells from the left panel were pregated as live cells, singlet cells positive for human CD45. ROR1⁺CD19⁺ cells were further analyzed for their expression of CD27, IgD, CD10, CD5, and CD38. Representative data from 3 different cohorts of huNSG mice are shown. (b) Splenocytes from huNSG mice were stained with CellTrace Violet and stimulated with PWM, CpG, and SAC or left unstimulated. After 4 days, cells were harvested and stained with CD19 and ROR1 antibodies, and percentage of proliferation of live gated CD19 B cells was measured by dye dilution by flow cytometry. The % of proliferating ROR1⁺ and ROR1⁻ B cells is shown. The graph depicts the results from 2 experiments.

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