CTLA4Ig Improves Murine iTreg Induction via TGF<i>β</i> and Suppressor Function <i>In Vitro</i>

<div>Suppressive potential of <i>in vitro</i> induced iTregs is not impaired by CTLA4Ig. For <i>in vitro</i> suppressor assays, titrated numbers of Treg-enriched cells (after cultivation with TGF<i>β</i> ± CTLA4Ig; Teff : Tregs) were added to 4 × 10<sup>6</sup> naïve B6 responder cells (responder cells: Tregs). Responder cells were stimulated with (a) 4 × 10<sup>6</sup> fully allogeneic BALB/C stimulator cells (irradiated) or (b) by polyclonal stimulation with plate-bound anti-CD3. Stimulation indices (SI; calculated in at least triplicates divided by pooled medium controls) of coculture suppression by Tregs induced in the presence of CTLA4Ig were compared to TGF<i>β</i> Treg controls. 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TGF<i>β</i> Tregs w/o CTLA4Ig.</div>

Journal of Immunology Research

fig3

Figure 3

Figure 3: CTLA4Ig Improves Murine iTreg Induction via TGF<i>β</i> and Suppressor Function <i>In Vitro</i> 

Journal of Immunology Research

CTLA4Ig Improves Murine iTreg Induction via TGFβ and Suppressor Function In Vitro

Figure 3